Staphyt’s laboratory assists you in testing, evaluating and screening biostimulants before and after registration, under controlled and semi-controlled conditions.
Currently, conventional agronomic assessments (height, yield, plants architecture…) measured in field or in laboratory (under controlled conditions), provide indications regarding the products’ effect on the plant physiology but need to be reinforced through innovating approaches.
The laboratory in Martillac (France) seeks to develop molecular biology tools to comfort the efficacy of biostimulants by analyzing the activation of intrinsic mechanisms to the plant such as the transport or metabolism of nutrients.
Phosphorus deficiency is one of the main limiting factors for plant growth and development. Despite its abundance in the soil in organic or inorganic form, only a small part is bioavailable and absorbable by the roots. Regular applications of fertilizers are then necessary to maintain high yields.
We are developing a test to evaluate the effect of biostimulants under phosphorus deficiency conditions in wheat and tomato.
Firstly, targeted marker genes, whose expression is linked to phosphorus transport, were identified, and then, the products’ capacity to modulate these selected genes’ expression was evaluated by RT-qPCR.
For that purpose, an in silico meta-analysis was developed to generate specific primers. This methodology is based on the alignment of the multiple sequences available for a marker gene described in the literature and the comparative use of different primer analysis softwares. Therefore, primers could be designed for each gene and species.
This overall strategy could be further extended to the evaluation of biostimulants against other abiotic stresses such as water stress.
The first results of RT-qPCR analyses performed on biostimulated and/or phosphorus-deficient plants will be presented and compared to conventional agronomic analyses at Plant BioProtech 2022.
For more information, do not hesitate to contact us