New molecular biology platform: new service offers

Staphyt’s laboratory (Martillac, France) has been supporting you for 30 years in selecting, testing and evaluating your active substances and formulated products, upstream and downstream of approval.

In connection with the development of R&D at Staphyt, the laboratory is now going further by equipping itself with a molecular biology platform. These new tools enable us to complete our analysis system and strengthen our range of services in 4 main areas:

1 / DETECTION AND QUANTIFICATION OF RESISTANCES

FUNGICIDE RESISTANCE

In order to prevent the appearance of resistance and thus prolong the products’ efficacy in the fields, it is necessary to monitor the populations of pathogens and thus verify their proper response to the products. When this resistance is proven, quantitative monitoring of this resistance is set up to follow its evolution.

This monitoring is usually carried out by our team with in vivo tests. Today the process is completed by molecular biology tools, such as sequencing and q-PCR which allow both the identification and the detection of mutations at the origin of these resistances but also the quantification of these resistances. 

Thus the laboratory is already able to monitor the strain sensitivity of different pathogens (Plasmorapa viticola, Erysiphe necator (downy mildew, powdery mildew, sclerotinia), Septoria tritici (septoria leaf blotch)…) when exposed to different active ingredients as CAA, QOI QiI, SdHI …. Other tests are also available on request.

HERBICIDE RESISTANCE
Resistance mechanisms are also observed for herbicide products with the emergence of weeds that are not controlled by the use of these compounds. The laboratory is now able to detect resistance to these products.

2 / DETECTION AND QUANTIFICATION OF PLANT DEFENSE ACTIVATION

A new pathogen control strategy consists in using PDS (plant defense stimulators) which are biocontrol agents that trigger the activation of plant defense systems.

Placing these products on the market requires demonstrating, on the one hand, their no or low direct biocidal activity and, on the other hand, their ability to activate plant defense mechanisms.

For 5 years now, the laboratory has been offering an in vitro screening test GUSTAVE (for GUS Technology for the Analysis and Validation of your plant Elicitor) which makes it possible to easily assess (white/blue test) the ability of a product to activate plant defenses and determine the defense pathway involved.

In addition to this test, we now use technologies that enable us to quantify the level of expression of marker genes that have been selected by our teams to reflect the level of activation of plant defense pathways. These tools are already available on various plants of interest (Arabidopsis, Brachypodium, Seedrape, Tomato, Vine, etc.).

3 / DEMONSTRATION OF THE ACTIVITY OF BIOSTIMULANT PRODUCTS BY ACTIVATION OF GENES INVOLVED IN NUTRITION OR IN RESISTANCE TO ABIOTIC STRESS

Biostimulants are products that activate the metabolic capacities of plants.

Currently, the results obtained in the field and in the laboratory (tests under controlled conditions) via conventional agronomic measurements provide indications  about the as to the product’s effect on plant physiology. These observations are not always able to statistically demonstrate their efficacy.

Molecular biology tools then support the evaluation of biostimulants and demonstrate their efficacy by activating metabolic pathways.

We are already able to perform this type of analyses for the metabolic pathways involved in water stress and phosphorus deficiency resistance.

4 / CUSTOMIZED TESTS / ADVICE / EXPERTISE

Staphy supports you in your innovation projects in plant protection and nutrition.

Attentive to your needs, we offer our expertise to provide you with innovative solutions in molecular biology and develop tailor-made R&D according to your expectations and needs (scientific and technological watch and design of protocols specifically adapted to your projects).

Our experts support you in particular with your projects that involve sequencing, PCR, RT-qPCR type analyses, but also on larger-scale projects through high-throughput sequencing analyses (NGS, new generation sequencing).

For more information, do not hesitate to contact us.