Molecular Biology : new tools for new services!

Through the deployment of R&D at Staphyt, our laboratory seeks to complete its range of services using Molecular Biology technics, especially on 3 main topics:

1 / Fungicide Resistance: faster and reliable detection with molecular biology technics

Many studies have allowed progress in advances in the understanding of the molecular mechanisms of fungicide resistance. These studies have led to the development of fast and effective methods for the detection and quantification of resistant genotypes. These techniques now provide an interesting, promising alternative to conventional bioassays which although reliable / robust are labor-intensive and time-consuming if large numbers of isolates are to be tested.

Staphyt has developed molecular biology tools, as PCR and Q-PCR  in order to detect and follow the emergence of fungicide resistance in several pathogens. We are able to detect the different strains of Plasmopara viticola resistant to CAA (3 different genotypes) and to quantify the resistant genes to the QoI fungicides. We are also developing methods of detection of strains of Septoria triticii resistant to SDHI.

2/ Pathogen detection: specific and accurate molecular biology method

Xanthomonas arboricola pv. juglandis is the causal agent of walnut blight. This disease causes severe crop damages and lead to a significant  loss of nut’s harvest (about 50%). Moreover, the emergence of copper resistant populations makes the authorized chemical treatment inefficient, in order to control the disease and to clean up the orchards, a specific and sensitive detection method is available :

A duplex PCR method enables us to detect the presence of the bacteria  Xanthomonas arboricola juglandis in dormant walnut buds. Subsequently, analysis can be performed on these detected bacteria to test their sensitivity to copper through in vitro test.

3 / Detection of the defense gene activation: demonstrate your product performance within Molecular Biology method

An emerging strategy of pest control is to use compounds that elicit plant defenses, Elicitors are considered as promising biological control tools and draw major interest in IPM strategies.

For the validation of a product’s elicitor activity by the authorities, one aspect is to both accurately demonstrate, its ability to stimulate the plant defense mechanism and its non-fungicidal nature.

GustaveGustave (GUS Technology for Analysis  and Validate Plant Elicitor) is one system to evaluate the elicitor activity of a product. Recently, Staphyt has developed a quantitative method, using BioMolecular method, providing relative expressions of defense genes (SAR and ISR pathways marker’ genes). Hence, it makes it possible to follow the activation of these genes expression when the product is applied, in order to demonstrate and quantify its elicitor activity.

For further information, don’t hesitate to contact us.